On 19 April 2005, the Foundation approved the financing, in collaboration with the Royal Academy of History, of a series of lectures entitled "Doña Juana, Queen of Castile", directed by Professor Gonzalo Anes y Álvarez de Castrillón. The lectures took place between 25 November and 9 December 2005.
As pointed out in the conference programme ".The Royal Academy of History wishes to remember Isabella's heir, Princess Juana, as Queen of Castile. The reign of Doña Juana has deserved and deserves the attention of distinguished historians specialising in the study of events during the first half of the 16th century. Doña Juana, confined in Tordesillas from February 1509, met her death there on 12 April 1555, assisted by Saint Francisco de Borja.
Juana was soon removed from government duties, although she always retained her status as Queen. Her signature was unsuccessfully requested by the Comuneros in order to take legal action against King Charles I, her son. She was the mother of two emperors, Charles and Ferdinand, and of four queens: Eleanor of Portugal and France, Isabella of Denmark, Mary of Hungary and Catherine of Portugal. The personality of the queen, the circumstances in which she had to act and her time, were the subject of the series of conferences which shed light on what Doña Juana was and represented as a woman, still shrouded in legend, not without tenderness and nostalgia.".
The content of the conference took the form of the work "Doña Juana, Queen of Castile" published in the History Collection of the Rafael del Pino Foundation in 2006.